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Results of DNA assay used to determine the attachment of (A) BJ5ta cells and (B) MLOA5 cells onto PCL-only, PGS-M-only, and PCL-M:PGS-M porous scaffolds after 24 h compared to tissue culture plate monolayer (N = 3, n = 3, *P < 0.05 and ***P < 0.001). Results of resazurin assay from (C) BJ5ta cells and (D) MLOA5 cells seeded onto PCL-M-only, PGS-M-only, and PCL-M:PGS-M porous scaffolds on day 1, 4, and 8 (N = 3, n = 3; *P < 0.05, **P < 0.01, and ***P < 0.001). Representative water contact angle images of (E) bulk PCL-M, (F) bulk PGS-M, and (G) bulk 50:50 PCL-M:PGS-M.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Emulsion templating of PCL:PGS methacrylate blends for soft tissue engineering

doi: 10.3389/fbioe.2026.1758159

Figure Lengend Snippet: Results of DNA assay used to determine the attachment of (A) BJ5ta cells and (B) MLOA5 cells onto PCL-only, PGS-M-only, and PCL-M:PGS-M porous scaffolds after 24 h compared to tissue culture plate monolayer (N = 3, n = 3, *P < 0.05 and ***P < 0.001). Results of resazurin assay from (C) BJ5ta cells and (D) MLOA5 cells seeded onto PCL-M-only, PGS-M-only, and PCL-M:PGS-M porous scaffolds on day 1, 4, and 8 (N = 3, n = 3; *P < 0.05, **P < 0.01, and ***P < 0.001). Representative water contact angle images of (E) bulk PCL-M, (F) bulk PGS-M, and (G) bulk 50:50 PCL-M:PGS-M.

Article Snippet: Two cell lines were used to assess the biological properties of PCL-M-only, PGS-M-only, and PCL-M:PGS-M polyHIPE scaffolds: o MLOA5 cells - A late-stage murine osteoblast cell line (Kerafast, US). o BJ5ta cells - A human immortalised fibroblast cell line (ATCC, US).

Techniques: Resazurin Assay

(A) Comparison of Sirius red staining (SRS) on PCL-M:PGS-M scaffolds seeded with BJ5ta cells and MLOA5 cells vs. acellular scaffolds after 14 days of culture. (B) SRS absorbance readings on scaffolds vs. on tissue culture plastic (TCP). (N = 3, n = 3; *P < 0.05, **P < 0.01). For all readings, the background colour (acellular controls) was subtracted.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Emulsion templating of PCL:PGS methacrylate blends for soft tissue engineering

doi: 10.3389/fbioe.2026.1758159

Figure Lengend Snippet: (A) Comparison of Sirius red staining (SRS) on PCL-M:PGS-M scaffolds seeded with BJ5ta cells and MLOA5 cells vs. acellular scaffolds after 14 days of culture. (B) SRS absorbance readings on scaffolds vs. on tissue culture plastic (TCP). (N = 3, n = 3; *P < 0.05, **P < 0.01). For all readings, the background colour (acellular controls) was subtracted.

Article Snippet: Two cell lines were used to assess the biological properties of PCL-M-only, PGS-M-only, and PCL-M:PGS-M polyHIPE scaffolds: o MLOA5 cells - A late-stage murine osteoblast cell line (Kerafast, US). o BJ5ta cells - A human immortalised fibroblast cell line (ATCC, US).

Techniques: Comparison, Staining

Fluorescent staining (blue: DAPI, green: Phalloidin FITC) of (A) BJ5ta cells and (B) MLOA5 cells on day 4 of culture on porous PCL-M:PGS-M scaffolds. BJ5ta cells were observed to grow into the largest pores of the scaffold as shown by (C) the pore outline and (D) the depth of the cells within it.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Emulsion templating of PCL:PGS methacrylate blends for soft tissue engineering

doi: 10.3389/fbioe.2026.1758159

Figure Lengend Snippet: Fluorescent staining (blue: DAPI, green: Phalloidin FITC) of (A) BJ5ta cells and (B) MLOA5 cells on day 4 of culture on porous PCL-M:PGS-M scaffolds. BJ5ta cells were observed to grow into the largest pores of the scaffold as shown by (C) the pore outline and (D) the depth of the cells within it.

Article Snippet: Two cell lines were used to assess the biological properties of PCL-M-only, PGS-M-only, and PCL-M:PGS-M polyHIPE scaffolds: o MLOA5 cells - A late-stage murine osteoblast cell line (Kerafast, US). o BJ5ta cells - A human immortalised fibroblast cell line (ATCC, US).

Techniques: Staining

H&E staining of scaffold cross sections shows infiltration of BJ5ta and MLOA5 cells into PCL-M:PGS-M, PGS-M-only, and PCL-M-only polyHIPE scaffolds after 7 days of culture. Scale bars represent 250 μm. Magnification is ×10. Top of scaffolds (cell seeded surface) is indicated on images.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Emulsion templating of PCL:PGS methacrylate blends for soft tissue engineering

doi: 10.3389/fbioe.2026.1758159

Figure Lengend Snippet: H&E staining of scaffold cross sections shows infiltration of BJ5ta and MLOA5 cells into PCL-M:PGS-M, PGS-M-only, and PCL-M-only polyHIPE scaffolds after 7 days of culture. Scale bars represent 250 μm. Magnification is ×10. Top of scaffolds (cell seeded surface) is indicated on images.

Article Snippet: Two cell lines were used to assess the biological properties of PCL-M-only, PGS-M-only, and PCL-M:PGS-M polyHIPE scaffolds: o MLOA5 cells - A late-stage murine osteoblast cell line (Kerafast, US). o BJ5ta cells - A human immortalised fibroblast cell line (ATCC, US).

Techniques: Staining